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Tel: (404) 727-3729

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Emory University School of Medicine
615 Michael Street, Rm 323
Atlanta, GA 30322

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Tel: (404) 727-7564 (Main Lab)

(404) 712-0796

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FAX: (404) 727-5408

Department of Human Genetics
Emory University School of Medicine
615 Michael Street, Room 325.1 and 355
Atlanta, GA 30322

© 2019 - Peng Jin Lab

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Cytosine Modifications in Development and Stem Cells

Methylation of cytosine to form 5-methylcytosine (5mC) on genomic DNA plays important roles in regulating gene expression. Although DNA methylation has been well studied, the reverse process has only recently been revealed in mammalian cells. A group of iron (II)/αKG-dependent dioxygenases, the TET proteins, oxidize 5mC to 5-hydroxymethylcytosine (5hmC), and then to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). The resulting 5fC and 5caC can be recognized by mammalian DNA glycosylase, thymine DNA glycosylase (TDG), in a base excision repair process to convert back to cytosine, providing an active demethylation pathway.

 

In collaboration with Dr. Chuan He at U. Chicago, we developed a suite of new technologies to map the genome-wide distribution of these modified cytosines and study the active demethylation process. These methods provide the community the tools to investigate 5mC oxidation and active DNA demethylation.

Featured Papers

Song, C.X., Szulwach, K.E., Fu, Y., Dai, Q., Yi, C., Li, X., Li, Y., Chen, C.H., Zhang, W., Jian, X., Wang, J., Zhang, L., Looney, T.J., Zhang, B., Godley, L.A., Hicks, L.M., Lahn, B.T., Jin, P.*, and He, C*. (2010) A Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine. Nature Biotechnology, 29(1): 68-72. (*:Co-Corresponding authors) PMC3107705

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Yu, M., Hon, G.C., Szulwach, K.E., Song, C.X., Zhang, L., Kim, A., Li, X., Dai, Q., Shen, Y., Park, B., Min, J.H., Jin, P. *, Ren, B. *, and He. C. * (2012) Base-Resolution Analysis of 5-Hydroxymethylcytosine in the Mammalian Genome. Cell, 149: 1368-1380. (*: Co-Corresponding authors). PMC3589129

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Song, C.X., Szulwach, K.E., Dai, Q., Fu, Y., Mao, S.Q., Lin, L., Street, C., Li, Y., Poidevin, M., Wu, H., Gao, J., Liu, P., Li, L., Xu, G.L., Jin, P.*, and He, C.* Genome-wide Profiling of 5-Formylcytosine reveals its roles in epigenetic priming. Cell, 153: 678-691. (*:Co-Corresponding authors)  PMC3657391

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Wang, T., Wu, H., Li, Y., Szulwach, K.E., Lin, L., Li, X., Chen, I.P., Goldlust, I.S., Chamberlain, S.J., Ananiev, G., Mowrey, J., Han, J.W., Yoon, Y., Rudd, M.K., Song, C.X., He, C., Chang, Q., Warren, S.T., and Jin, P. (2013) Subtelomeric hotspots of aberrant 5-hydroxymethylcytosine-mediated epigenetic modifications during reprogramming to pluripotency. Nature Cell Biology, 15: 700-711.  PMC3998089

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